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KMID : 0378019700130120087
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New Medical Journal
1970 Volume.13 No. 12 p.87 ~ p.95
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Influences of the different growth temperatures of Ps. Aeruginosa upon the experimental mixed infections with Staphaureus
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Abstract
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Since the discovery and wide application of antibiotic therapy, the pattern of microbial infections has been markedly changed. In the surgical field, cases of mixed bacterial infections, particuarly those of Ps. aeruginosa and Staph. aureus, are increasing and have been studied by some workers. However, the precise mechanisms are rot well understood.
The author conceives of possible influences of different growth temperatures of Ps. aerugirosa upon the course of experimental mixed infection with Staph. aureus. Thus, a strain of Ps. aeruginosa was cultivated at 3 different growth temperatures, namely 30°C, 37°C, and 40°C, and successive subcultures were made at the same temperatures. After more than 10 subcultures, cultures of Ps. aerugirosa were mixed with cultures of Staph. aureus and inoculated into the ear veins of rabbits. Heart blood and bladder urine were taken aseptically after 3 hours, and 1, 2, 3, 5 and 7 days of infection, and the viable number of organisms in the specimens was determined by cultivating serial dilutions and using pour-plate method.
The results were summarized as followed:
1. When the cultures of Staph. aureus and Ps. aeruginosa grown at 30°C or 37°C were mixed and inoculated, viable units of both species in the blood stream decreased rapidly and could not be recovered after 2-3 days.
2. When the cultures of Staph. aureus and Ps. aeruginora grown at 40°C were mixed and inoculated, viable units of both species in the blood stream decreased rapidly, but small number of both species could be recovered occasionally through 7 days of infections.
3. When the pseudomonas culture at 30°C and staphylococci were mixed and inoculated, the viable units of staphylococci in the urine increased sharply until around the 3rd day of infection and then decreased. After relatively small number until the 2nd day, the pseudomonas in the urine decreased sharply and became almost not recoverable after 3 days.
4. When the pseudomonas culture at 37°C and staphylococci were mixed and inoculated, the viable units of staphylococcus in the urine showed some decrease instead of increase during´ the period of 3 hrs. to 1-2 days, and then more or less gradual increase through 7 days of infection.
The pseudomonas in the urine increased slowly until around the 3rd . day and then showed no noticeable increase or decrease until the 7th day.
5. When pseudomonas culture at 40°C and staphylococci were mixed and inoculated, the viable units of staphylococci in the urine showed marked increase for 2-3 days without initial decrease, and then slow decrease or no changes in number. The viable units of pseudomonas in the urine showed rot much change for about 2 days and then increased until the 7th day.
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